Basophil activation test: food challenge in a test tube or specialist research tool?

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Basophil activation test: food challenge in a test tube or specialist research tool?

Oral food challenge (OFC) is the gold-standard to diagnose food allergy; however, it is a labour and resource-intensive procedure with the risk of causing an acute allergic reaction, which is potentially severe. Therefore, OFC are reserved for cases where the clinical history and the results of skin prick test and/or specific IgE do not confirm or exclude the diagnosis of food allergy. This is ...

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Basophil activation test is food adverse reactions

Methods 83 subjects (66/17 females/males) with reported food adverse reactions were included in the study. Eighteen atopic/allergic subjects were included as controls for the foods to which they had reported no reactions. BAT was performed on heparinazed blood incubated with food extracts. CD63 activation marker was evaluated on basophils by flow-cytometry. The stimulation index (SI) was calcul...

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Basophil Activation Test with Food Additives in Chronic Urticaria Patients

The role of food additives in chronic urticaria (CU) is still under investigation. In this study, we aimed to explore the association between food additives and CU by using the basophil activation test (BAT). The BAT using 15 common food additives was performed for 15 patients with CU who had a history of recurrent urticarial aggravation following intake of various foods without a definite food...

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Basophil activation test in allergic rhinitis.

903 a different perception on rhinitis, be it caused by an IgEor a non -IgE -mediated challenge, because CD63 is easily activated by nonallergic stimulation.4 Furthermore, the ability to perform an accurate BAT depends also on the gating strategy used by the test to catch basophils in the flow cytometry electronic capture.5 The Flow2CASTTM (BÜHLMANN, Schönenbuch, Switzerland) used by Leśniak et...

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Basophil activation test: handle with care.

The letter to the Editor by Dr Chirumbolo highlights the major pitfalls in basophil activation test (BAT) performance. In my view, basophil activation test should be considered as an additional clinical tool in the diagnosis of allergy and not the “absolute truth”. Basophils play a pivotal role as effector cells in allergic disease, despite representing a small population of peripheral leucocyt...

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ژورنال

عنوان ژورنال: Clinical and Translational Allergy

سال: 2016

ISSN: 2045-7022

DOI: 10.1186/s13601-016-0098-7